Connect with us

Bioengineer

The CNIO reprograms CRISPR system in mice to eliminate tumor cells without affecting healthy cells

Credit: CNIO The CRISPR/Cas9 gene-editing tool is one of the most promising approaches to advancing treatments of genetic diseases -…

Published

on

The CRISPR/Cas9 gene-editing tool is one of the most promising approaches to advancing treatments of genetic diseases – including cancer -, an area of research where progress is constantly being made. Now, the Molecular Cytogenetics Unit led by Sandra Rodríguez-Perales at the Spanish National Cancer Research Centre (CNIO) has taken a step forward by effectively applying this technology to eliminate so-called fusion genes, which in the future could open the door to the development of cancer therapies that specifically destroy tumours without affecting healthy cells. The paper is published in Nature Communications.

Fusion genes are the abnormal result of an incorrect joining of DNA fragments that come from two different genes, an event that occurs by accident during the process of cell division. If the cell cannot benefit from this error, it will die and the fusion genes will be eliminated. But when the error results in a reproductive or survival advantage, the carrier cell will multiply and the fusion genes and the proteins they encode thus become an event triggering tumour formation. “Many chromosomal rearrangements and the fusion genes they produce are at the origin of childhood sarcomas and leukaemias,” explains Sandra Rodríguez-Perales, lead co-author of the study now published by the CNIO. Fusion genes are also found in among others prostate, breast, lung and brain tumours: in total, in up to 20% of all cancers.

Because they are only present in tumour cells, fusion genes attract a great deal of interest among the scientific community because they are highly specific therapeutic targets, and attacking them only affects the tumour and has no effect on healthy cells.

And this is where the CRISPR technology comes into play. With this technology, researchers can target specific sequences of the genome and, as if using molecular scissors, cut and paste DNA fragments and thus modify the genome in a controlled way. In the study carried out by the CNIO team, the researchers worked with cell lines and mouse models of Ewing’s sarcoma and chronic myeloid leukaemia, in which they managed to eliminate the tumour cells by cutting out the fusion genes causing the tumour.

The tumour cell repairs itself… and destroys itself

This is the first time that CRISPR has been successfully applied for the selective elimination of fusion genes in tumour cells. Earlier strategies by other research teams were based on modifying the junction between the two genes involved in the fusion to introduce a DNA sequence that induces cell death. The problem is that the introduction of foreign sequences has proven to be very ineffective in eliminating tumours.

The CNIO researchers used a completely different approach to induce the tumour cell to destroy itself. “Our strategy was to make two cuts in introns, non-coding regions of a gene, located at both ends of the fusion gene,” explains Raúl Torres-Ruiz, co-author of the paper. “In that way, in trying to repair those breaks on its own, the cell will join the cut ends which will result in the complete elimination of the fusion gene located in the middle”. As this gene is essential for the survival of the cell, this repair automatically causes the death of the tumour cell.

“Our next steps will be to carry out more studies to analyse the safety and efficiency of our approach,” continues Rodríguez-Perales. “These steps are essential to know if our approach can be translated in the future into a potential clinical treatment. Furthermore, we will study whether our strategy, which we have already seen works in Ewing’s sarcoma and chronic myeloid leukaemia, is also effective in other types of cancer caused by fusion genes and for which there are currently no effective therapies,” he concludes.

###

The study was funded by the Ministry of Science and Innovation, the Carlos III Health Institute, the European Regional Development Fund, the Spanish Association against Cancer (AECC), the European Research Council, the Catalan Government, the Cell Therapy Network, FERO, the “la Caixa” Foundation, the José Carreras Foundation and the Xarxa de Bancs de Tumors de Catalunya.

https://www.cnio.es/en/news/publications/reprogramming-crispr-system-in-mice-to-eliminate-tumour-cells-without-affecting-healthy-cells/

Source: https://bioengineer.org/the-cnio-reprograms-crispr-system-in-mice-to-eliminate-tumor-cells-without-affecting-healthy-cells/

[ALT0]

0 Users (0 votes)
Criterion 1
What people say... Leave your rating
Sort by:

Be the first to leave a review.

User Avatar
Verified
{{{ review.rating_title }}}
{{{review.rating_comment | nl2br}}}

Show more
{{ pageNumber+1 }}
Leave your rating

Continue Reading
Click to comment

Leave a Reply

Your email address will not be published. Required fields are marked *

Title

Uncategorized2 mins ago

Revain REV

0 Users (0 votes) Criterion 1 What people say... Leave your rating Sort by: Most recent Top score Most helpful...

Coinpedia2 hours ago

Top 3 Reasons, Why XRP Price is Pumping very hard

XRP price has become the talk of the town in recent days with remarkable gains within a short stipulated time....

Coinpedia10 hours ago

Ethereum Price Analysis, ETH Price May Break Above $1K by Dec 2020?

As the ETH price surged, a huge mass of crypto enthusiasts shifted their focus from the other cryptos to Ethereum....

Bioengineer13 hours ago

Успешный краш-тест соответствует важной вехе в программе ядерного сдерживания

Команда Sandia работала над решением проблем COVID-19 и своевременно добилась результатов Фото: Фото Брета Латтера, Sandia National Laboratories, Альбукерке, Нью-Мексико…

Coinpedia14 hours ago

DOT to Outperform ETH Capturing its Big Portion of Market Capitalization

One of the popular crypto analyst has predicted the DOT ecosystem will outperform the ETH platform in the coming days....

Business insider14 hours ago

Clean Power Capital Announces Appointment of Leading Clean Energy Entrepreneur Greg Nuttall to the PowerTap Advisory Board | Markets Insider

VANCOUVER, British Columbia, Nov. 23, 2020 (GLOBE NEWSWIRE) -- Clean Power Capital Corp. (CSE: MOVE)(FWB: 2K6)(OTC: MOTNF) (“Clean Power” or...

Bioengineer1 day ago

Successful crash test meets major milestone for nuclear deterrence program

Sandia team worked through COVID-19 challenges, delivered results on timeCredit: Photo by Bret Latter, Sandia National Laboratories ALBUQUERQUE, N.M. --...

Blockchain news1 day ago

ETH, XRP, BCH See Bullish Gains, But Still a Far Cry from All-Time Highs

Bitcoin and the top altcoins ETH, XRP, and BCH has seen impressive surges in recent times but the latter three...

Entrepreneur1 day ago

3 Ways You Can Level the Playing Field Against Big Business

To gain ground against well-established rivals, focus on the three Cs: Competition, credit and connections....

Techcrunch1 day ago

A bug meant Twitter Fleets could still be seen after they disappeared – TechCrunch

Fleets live on Twitter's servers for up to 30 days....

Review

    Select language